中国兽医科学2013，Vol.43Issue(4)：401-406,6.
鹅干扰素-α的毕赤酵母表达及其生物学活性分析
Expression of goose interferon-alpha in Pichia pastoris and analysis of its antiviral activity
摘要
Abstract
In order to get alpha-interferon( IFN-α) with high level secretive expression and antiviral activities, the goose IFN-α gene and the α-factor signal sequence of pPICZaA were amplified by fusion PCR. The target gene and pPICZαA were digested with HindIII + Kpn I and then were ligated. The recombinant plasmid of pPICZaA- GoIFN-α was linearized by Sac I and electrotratnsformation into Pichia pastoris X-33. PCR assay was used to identify colonies. The positive recombinants were screened and induced by addition of methanol. GoIFN-α protein was detected by SDS-PAGE and Western-blotting analyses. The result showed the IFN-α protein with a molecular mass of 25 — 35 ku was expressed in Pichia pastoris X-33. The antiviral activity of IFN-α against GPMV was investigated on the GEF cell and the result indicated that IFN-α could inhibit GPMV.关键词
鹅/干扰素-α/巴斯德毕赤酵母/分泌表达/抗病毒活性Key words
goose/interferon-alpha/Pichia pastoris/secrete expression/anti virus activity分类
农业科技引用本文复制引用
朱永梅,曹永生,李鑫,马波,张文龙,高明春,王君伟..鹅干扰素-α的毕赤酵母表达及其生物学活性分析[J].中国兽医科学,2013,43(4):401-406,6.基金项目
黑龙江省十二五科技攻关项目(GA09B302) （GA09B302）
