中国兽医科学2013，Vol.43Issue(4)：412-415,4.
牛瑟氏泰勒虫与牛卵形巴贝斯虫多重PCR检测方法的建立
Establishment of multiplex PCR for detection of bovine Theileria sergenti and Babesia ovata
摘要
Abstract
For simultaneous detection of Theileria sergenti and Babesia ovata ,a multiplex PCR was established using 2 pairs of specific primers designed on the basis of ITS gene of Theileria sergenti and CCTη gene of Babesia ovata. Reaction conditions of the multiplex PCR were optimized. Two targeting fragments, 1 020 bp(for T. sergenti) and 537 bp for (B. ovata), were amplified. The detection limit was 10 pg/μL for T. sergenti and 1 pg/μL for B. ovata, respectively. Twenty-three clinical samples from Hunchun in Jilin province were detected by the multiplex PCR and the detection rate was 69% for T. sergenti; 52% for B. ovata and 52% for mixed infection. This method could be useful in clinic diagnoses.关键词
牛瑟氏泰勒虫/牛卵形巴贝斯虫/多重PCRKey words
Theileria sergenti/Babesia ovata/multiplex PCR分类
农业科技引用本文复制引用
王轶男,钱年超,黄国明,胡诗悦,薛书江,贾立军,张守发..牛瑟氏泰勒虫与牛卵形巴贝斯虫多重PCR检测方法的建立[J].中国兽医科学,2013,43(4):412-415,4.基金项目
国家自然科学基金项目(30960278) （30960278）
吉林省自然科学基金资助项目(201115230) （201115230）
