中国畜牧兽医2013，Vol.40Issue(4)：22-25,4.
犬瘟热病毒截短P蛋白单克隆抗体的制备与鉴定
Preparation and Identification of Monoclonal Antibodies against Recombinant Truncated P Protein of Canine Distemper Virus
摘要
Abstract
The assay was aimed to prepare monoclonal antibodies (McAbs) against P1 protein of canine distemper virus (CDV). BALB/c mice were immunized with P1 protein and the immunized mice spleen myeloma cells were fused with SP2/0. Two hybridoma cell lines of E9E4C10 and E9F8D9 that stably produced antibodies against protein were identified by ELISA. The antibody titres of ascites for the hybridoma lines were both 1:106. The subtypes of monoclonal antibodies were both identified as IgG2b. Western blotting and IFA showed that the McAbs specifically recognized certain antigenic epitopes of P1 protein of CDV. Additive index of ELISA competitive binding assay indicated that the two monoclonal antibodies were specific for different epitopes. The McAbs prepared in this study were useful tools for developing immunological diagnosis techniques of CDV and investing P protein function.关键词
犬瘟热病毒/重组P蛋白/单克隆抗体/鉴定Key words
canine distemper virus/ recombinant P protein/ monoclonal antibodies/ identification分类
农业科技引用本文复制引用
袁维峰,贾红,于萍萍,侯绍华,郭晓宇,史利军,赵占中,朱鸿飞..犬瘟热病毒截短P蛋白单克隆抗体的制备与鉴定[J].中国畜牧兽医,2013,40(4):22-25,4.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(2011js-3、2010js-1、2010js-2) （2011js-3、2010js-1、2010js-2）
2012年农业部动物疫情监测与防治项目. （）
