中国畜牧兽医2013，Vol.40Issue(4)：36-39,4.
狂犬病病毒BD-06株基质蛋白的原核表达、纯化及其多克隆抗体的制备
Prokaryotic Expression and Purification of Matrix Protein of BD-06 Strain Rabies Virus and Preparation of its Polyclonal Antibody
摘要
Abstract
The objective of this study was to obtain polyclonal antibody against the matrix protein of rabies virus in order to provide materials for further study on the function of matrix protein. The full sequence of the matrix protein gene from the BD-06 strain rabies virus was successfully cloned and inserted into the expression vector pET28a. The results of restriction enzyme digesting and sequencing proved that the matrix protein gene was correctly inserted into pET28a. After the expression plasmid was transformed into E. coli Rosetta and induced by 0. 5 mmol/L IPTG, 27 ku M protein was obtained. After the matrix protein was purified and vaccinated the rabbit, the polyclonal antibody was obtained. The results of Western blotting and IFA showed that the antibody could react with the rabies virus. The matrix protein in the BHK-21 cell affected by BD-06 strain rabies virus was positioned by using the polyclonal antibody. The results indicated that the polyclonal antibody against the matrix protein of rabies virus was successfully prepared, which laid a foundation for further study on matrix protein of rabies virus.关键词
狂犬病病毒/M蛋白/原核表达/多克隆抗体Key words
rabies virus/ matrix protein/ prokaryotic expression/ polyclonal antibody分类
生物科学引用本文复制引用
赵雪超,张学炜,张守峰,刘晔,米立娟,扈荣良..狂犬病病毒BD-06株基质蛋白的原核表达、纯化及其多克隆抗体的制备[J].中国畜牧兽医,2013,40(4):36-39,4.基金项目
国家"863"计划项目支助(201203056) （201203056）
国家公益性(农业)行业专项(2012AA100505). （农业）
