中国畜牧兽医2013，Vol.40Issue(4)：59-64,6.
牦牛乳脂合成关键酶基因全泌乳期的表达研究
Study on Expression of Key Enzyme Genes Related to Milk Fat Synthesis in Mammary Gland of Yak
摘要
Abstract
The Maiwa yak, which were used as milk producer in the northwest plateau of Sichuan province, were selected as experimental animal. The Real-time quantitive (RT-PCR) was applied to detect the expression level of ACSL1, ACSS1, ACSS2 and FABP3 genes in the mammary gland of yak during the lactation cycle. The results showed that ACSL1, ACSS1, ACSS2 and FABP3 gene were consistently expressed during the whole lactation period. RT-PCR results showed that the expression level of all 4 genes consistently increased from time point -15 d, and the peak appeared at time point 30 d. The expression level of all 4 genes at 30 d significantly increased (P<0. 05) compared with time point -15 d. Meanwhile, the peak of milk yield was at time point 120, 90 d later than the peak of mRNA expression levels, then the milk yield consistently decreased till 240 d. These results implied that the expression pattern of milk fat synthesis gene during the lactation cycle reflected the yak's genetic adaption to living environment in Tibetan plateau.关键词
麦洼牦牛/乳脂合成关键酶基因/乳腺/实时荧光定量PCRKey words
Maiwa yak/milk fat synthetic key enzyme genes/mammary gland/Real-time PCR分类
生物科学引用本文复制引用
李钰璨,邓霄禹,江明锋..牦牛乳脂合成关键酶基因全泌乳期的表达研究[J].中国畜牧兽医,2013,40(4):59-64,6.基金项目
四川省青年科技基金(09ZQ026-011) （09ZQ026-011）
自然科学基金(31172198) （31172198）
教育部科学技术研究重点项目(210264) （210264）
2011年度留学人员科技活动项目择优资助. （）
