中国畜牧兽医2013，Vol.40Issue(4)：69-72,4.
猪传染性胸膜肺炎放线杆菌PCR诊断方法的建立与应用
Establishment and Application of PCR Method for Diagnosing Actinobacillus pleuropneumoniae
摘要
Abstract
According to the APP included in GenBank serum surface antigen of D15 type 1 to 14 and 16S rRNA gene sequences were designed two pairs of primers, namely amplified two nucleotide fragments of 637, 431 bp, and built PCR detection method of APP. The results indicated that type-specific serum 1, 3, 5a, 8, 10 could amplify two expected fragments and Escherichia coli, Pasteurella, Salmonella, Streptococci and Staphylococci, and they were negative. Sensitivity checked out test results showed that the lowest concentration was 50/μL. 5 strains of clinical isolates strain suspected APP by PCR ,the results showed that in four of them were positive, one was negative. The specificity of PCR detection method of the experimental establishment was strong specificity, high sensitivity.关键词
猪传染性胸膜肺炎/D15基因/16S rRNA/聚合酶链式反应(PCR)Key words
porcine contagious pleuropneumonia/D15 gene/16S rRNA/polymerase chain reaction(PCR)分类
农业科技引用本文复制引用
张建新,朱岩昆,马金友,赵杰,余燕,韩科芳..猪传染性胸膜肺炎放线杆菌PCR诊断方法的建立与应用[J].中国畜牧兽医,2013,40(4):69-72,4.基金项目
河南省基础前沿与技术项目(1023000410016) （1023000410016）
河南省科技攻关项目(092102110161). （092102110161）
