中国畜牧兽医2013，Vol.40Issue(4)：81-84,4.
羊毛尾线虫线粒体nad4基因的序列变异分析
Analysis of Sequence Variation in Mitochondrial nad4 Gene of Trichuris ovis Isolates
摘要
Abstract
The objectives of the present study were to analyze sequence variation in the NADH dehydrogenase subunit 4 (nad4) gene among Trichuris ovis isolates from Guangdong province in China, and to study its phylogenetic relationships with other whipworms using the nad4 gene sequences. The partial nad4 (pnad4) were amplified from individual T. ovis samples, and pnad4 sequences were aligned using the ClustalX 1. 81. Neighbor-joining (NJ) trees were constructed using the software Mega version 5. 0. The lengths of all pnad4 sequences were 827 bp. Phylogenetic analyses showed that all T. ovis isolates were clustered in the same clade. Sequence variations in pnad4 sequences within T. ovis were 0 to 1. 5% , which was significantly lower than inter-species differences (37. 8% to 45. 6%). ft was concluded that pnad4 sequence could be used as a genetic marker for population genetic studies of T. ovis, as well as for the differentiation and identification of different whipworms. The results of this study provided foundation for further studies on molecular genetics and diagnostics of T. ovis.关键词
羊鞭虫/线粒体DNA/nad4基因/种系发育关系Key words
Trichuris ovis/ mitochondrial DNA/NADH dehydrogenase subunit 4 gene/ phylogenetic relationship分类
生物科学引用本文复制引用
王燕,杨言川,张悦文,李佳缘,朱兴全,刘国华..羊毛尾线虫线粒体nad4基因的序列变异分析[J].中国畜牧兽医,2013,40(4):81-84,4.基金项目
家畜疫病病原生物学国家重点实验室开放基金(SKLVEB2010KFKT010、SKLVEB2011KFKT004) （SKLVEB2010KFKT010、SKLVEB2011KFKT004）
国家肉牛牦牛产业技术体系项目(CARS-38). （CARS-38）
