中国畜牧兽医2013，Vol.40Issue(4)：89-92,4.
猪繁殖与呼吸综合征病毒SYBR Green Ⅰ荧光定量PCR检测方法的建立
Establishment of SYBR Green Ⅰ Fluorescent Quantitative PCR Method for Detection of Porcine Reproductive and Respiratory Syndrome Virus
摘要
Abstract
A pair of specific primers conserved in the the Nsp7 genes of American porcine reproductive and respiratory syndrome virus (PRRSV) were synthesized. Optimization of reaction conditions, the detection of SYBR Green Ⅰ Real-time quantitative PCR method was compared and analyzed at the same time with the commercialization of fluorescent quantitative PCR kit. The results showed that the specificity of the method, high sensitivity, a good distinction between the American swine reproductive and respiratory syndrome virus and other viruses. 14 clinical samples were detected using this method, the positive rate was 85. 7%, coincidence was 100% with commercial kits.关键词
猪繁殖与呼吸综合征病毒/实时荧光定量PCR/Nsp7Key words
porcine reproductive and respiratory syndrome virus/ Real-time quantitative PCR/Nsp7分类
农业科技引用本文复制引用
黄震,魏春娅,闫明菲,田悦茹,张民泽,谢杰雄,孙龙,张桂红..猪繁殖与呼吸综合征病毒SYBR Green Ⅰ荧光定量PCR检测方法的建立[J].中国畜牧兽医,2013,40(4):89-92,4.基金项目
国家生猪现代农业产业体系(nycytx009). （nycytx009）
