中国畜牧兽医2013，Vol.40Issue(4)：134-137,4.
荷斯坦奶牛子宫内膜上皮细胞的体外培养
Cell Culture of Endometrial Epithelial Cells from Chinese Holstein Dairy Cows in vitro
摘要
Abstract
To establish and perfect cell culture technique of endometrial epithelial cell (EnEpC)in dairy cows, primary cell was isolated and cultured by tissue cultivation that combined with enzymolysis of collagenase. The endometrial epithelial cell was purified from primary culture cells by different time digestion of trypsase, and EnEpC was identified by immunohistochem-ical detection of cytokeratin expression. The results demonstrated that flagstone-like epithelial cell was obtained by the adoptive method, and cytokeratin expression was positive in the cells. The purified cells could be subcultured about 10 times, and cells from generation 2 to 8 maintained stably biological characteristics of morphology and proliferation state that were found in primary culture cells.关键词
子宫内膜上皮细胞/细胞培养/荷斯坦奶牛Key words
endometrial epithelial cell/cell culture/Holstein dairy cows分类
生物科学引用本文复制引用
张世栋,王东升,王旭荣,潘虎,李宏胜,严作廷..荷斯坦奶牛子宫内膜上皮细胞的体外培养[J].中国畜牧兽医,2013,40(4):134-137,4.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(1610322012004) （1610322012004）
"十二五"国家科技支撑计划重点课题(2012BAD12B03). （2012BAD12B03）
