中国循证儿科杂志2013，Vol.8Issue(2)：98-104,7.DOI:10.3969/j.issn.1673-5501.2013.02.004
2008至2011年上海单中心住院腹泻儿童轮状病毒基因型别流行特征
Epidemical characteries of group A Rotavirus genotypes in children hospitalized with diarrhea in Shanghai, during 2008-2011
摘要
Abstract
Objective This shirty aimed to analyze the dynamic characteristics of group A rotavirus genotypes. Methods A total of 345 rotavirus-positive stool specimens were collected from inpatients under 5 years old hospitalized with acute gastroenteritis in Children's Hospital of Fudan University from January 2008 to December 2011. Each fecal specimen was tested for rotavirus with a commercial enzyme immunoassay, and genotypes were characterized using multiplex nested reverse transcription polymerase chain reaction ( RT-PCR ). Results G genotypes: The predominant G genotype was G3 which accounted for 49. 2% , 44. 6% and 78. 0% respectively in the year of 2008, 2009 and 2010. The prevalence of G9 was on an upward trend from 2008 to 2011, and dominated as the most common type in 2011( 51.1%). G1 was detected each year with a varying prevalence of 8% - 20% . G2 was rarely seen and G4 was not detected during the four years. Mixed G infections with G3 + G9 were the most prevailing G-mixed types, followed by G3 + G1. Otherwise, mixed-G infections with three different G genotypes were detected in 4 cases. P genotypes: P[ 8 ] genotype was the dominant strain with prevalence of 55. 6% , 60. 0% and 68. 1% respectively in the year of 2008 , 2010 and 2011, whereas mixed P genotype was dominant in 2009( 43. 2% ). P[ 4 ] strains were prevalent in four years in a volatility way. P [ 6 ] and P[ 9 ] could also be found as minor types. Interestingly, P[ 10 ] genotype was seen only in combination with P[ 8 ]. The major mixed P infection was genotype of P[ 4 ] + P[ 8 ], and P[ 8 ] + P[ 10 ] was assigned as follows. A high prevalence of P[ 8 ]G3 genotype was determined to be the major rotavirus genotype combination ( 24. 3% ) in Shanghai from 2008 to 2011 and the other prevalent genotypes included P[ 8 ]G9 and P[ m ]G3. P[ 8 ]G9 strains jumped as the most predominant type in 2011( 40. 5% ). Conclusions Compared with the data from the year of 2001 -2007, this study shows new epidemiological characteristics of group A rotavirus genotypes during 2008 - 2011 in Shanghai, including ascending of G1 and G9 proportions, reduction of G3 and diversification of mixed infections. Therefore, it is highly essential to conduct continuous and systematic monitoring for rotavirus strains in anticipation of rotavims vaccine introduction in Shanghai.关键词
婴幼儿/轮状病毒/腹泻/基因型Key words
Children/ Rotavims/ Diarrhea/ Genotype引用本文复制引用
卢丽娟,钟华清,苏犁云,曹凌峰,徐梦华,徐锦..2008至2011年上海单中心住院腹泻儿童轮状病毒基因型别流行特征[J].中国循证儿科杂志,2013,8(2):98-104,7.基金项目
新世纪优秀人才计划:NECT-09-0320 （）
上海市公共卫生优秀学科带头人计划:GWDTR201221 （）
