中国循证儿科杂志2013，Vol.8Issue(2)：149-153,5.DOI:10.3969/j.issn.1673-5501.2013.02.014
亚细亚酸对兔未成熟肺缺血再灌注损伤的保护作用
Asiatic acid attenuates ischemia-reperfusion induced lung injury in infant rabbits
摘要
Abstract
Objective The aim of the study was to examine whether asiatic acid could attenuate ischemia-reperfusion( I/R ) induced lung injury in infant rabbits. Methods 60 New Zealand white rabbits aged from 15 to 21 days were subjected to sham operation, I/R, low dose asiatic acid plus I/R, medium dose asiatic acid puls IR, and high dose asiatic acid puls I/R. I/R was induced by clamping the right pulmonary hilum for 1 hour and then removal of the clamp for 4 hours. Asiatic acid was given 3 days before operation by 7.5mg, 15, 30 mg · kg- 1respectively. Production and expression of free radical species-hydroxyl radical ( ROS-HR ), malondialdehyde ( MDA ), superoxide dismutase ( SOD ), glutathione peroxidase ( GSH-PX ), myeloid differentiation factor-88( MyD-88 ), and muclear factors-κB( NF-κB ) in the lung tissue were also evaluated. And temperature, hemodynamics, blood gases and neutrophil count were measured 2 hours after reperfusing. In addition, serum levels of interleukin-1 β and tumor necrosis factor-α were measured during the ischemia-reperfusion process. Results The infant lungs in asiatic acid middle and high dose groups had less neutrophil infiltration, edema, swelled alveolar epithelial and endothelial cells under light and electron microscopes. ②Compared wtih I/R group, asiatic acid reduced the production of ROS-HR and MDA and improved the activities of GSH-PX and SOD markedly, siginificantly decreased the serum levels of interleukin- 1β and tumor necrosis factor-α at 4 h time point after reperfusion, as well as the MyD88 and NF-κB of lung were siginificantly decreased( P <0. 05 ). ③Asiatic acid middle and high dose groups had good effect of attenuating I/R. Conclusions Asiatic acid pretreatment reduced the lung damage induced by I/R in the infant rabbits.关键词
幼兔/缺血再灌注/肺损伤/亚细亚酸Key words
Infant rabbit/ Ischemia-reperfusion/ Lung injury/ Asiatic acid引用本文复制引用
闫宪刚,仇万山,贾兵..亚细亚酸对兔未成熟肺缺血再灌注损伤的保护作用[J].中国循证儿科杂志,2013,8(2):149-153,5.基金项目
江苏省自然科学基金:BK2009452 （）
