中国药房2013，Vol.24Issue(16)：1517-1518,2.DOI:10.6039/j.issn.1001-0408.2013.16.24
HPLC-ELSD法测定盐酸氨基葡萄糖胶囊中主成分的含量
Content Determination of Glucosamine Hydrochloride Capsules by HPLC-ELSD
潘柏良 1刘霞2
作者信息
- 1. 重庆市中医院,重庆400011
- 2. 成都市高新区药工医药生物技术研究所,成都610041
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for the content determination of main component in Glucosamine hydrochloride capsules. METHODS: HPLC-ELSD method was adopted. The determination was performed on Apollo C18 column with mobile phase consisted of methanol-water-0.2 mol/L three fluorine acetic acid (5 : 90 : 50, V/V/V) at the flow rate of 0.7 ml/min. The column temperature was set at 30 ℃, and injection volume was 10 μl. The temperature of drift tube was 110 ℃, and the flow of carrier gas was 3.0 L/min. The response value was 1 and the impactor had been close down. RESULTS:The linear range of glucosamine hydrochloride was 34.6-346.0 μg/ml (r=0.999 8) with mean recovery of 99.0% (RSD=1.23% , n=9). CONCLUSION: The method is accurate, reliable, sensitive and reproducible, and it can be applied for the content determination of main components in Glucosamine hydrochloride capsules.关键词
盐酸氨基葡萄糖胶囊/高效液相色谱-蒸发光散射检测器法/含量测定Key words
Glucosamine hydrochloride capsules/ HPLC-ELSD/ Content determination分类
医药卫生引用本文复制引用
潘柏良,刘霞..HPLC-ELSD法测定盐酸氨基葡萄糖胶囊中主成分的含量[J].中国药房,2013,24(16):1517-1518,2.
