中国中医药信息杂志2013，Vol.20Issue(5)：32-34,3.DOI:10.3969/j.issn.1005-5304.2013.05.012
原花青素对视网膜缺血再灌注损伤大鼠视网膜结构及核转录因子-κB 表达的影响
Effect of Proanthocyanidins on Retinal Structure and NF-κB Expression on Retinal Ischemia-reperfusion Injury of Rats
摘要
Abstract
Objective To observe the effect of proanthocyanidins (PC) on retinal structure and NF-κB expression of retina ischemia-reperfusion (RIR) rats after injury, and explore its possible protection mechanisms. Methods All rats were randomly divided into five groups. In two weeks before modeling, PC low-, high-dose group were given 100, 300 mg/kg PC suspension, salvia miltiorrhiza group was given salvia miltiorrhiza particle solution 0.09 g/kg, normal group and model group were given normal saline, 1 time/d, and continued after modeling. Except for the normal group, other groups were divided into ischemia-reperfusion 6, 24, 48 and 72 h group. Immunohistochemistry methods were used to test retina NF-κB expression in each period, and ultrastructure of the retina was observed through the transmission electron microscope. Results The NF-κB began to express at 6 h after ischemia-reperfusion, expression was the strongest at 24 h, and then gradually decreased. NF-κB expression of PC low-, high-dose group and salvia miltiorrhiza group were significantly lower than that of the model group in the same period (P<0.01). The NF-κB expression of PC high-dose group was restrained significantly (P<0.01). By electron microscopy observation, 48 h model group ultrastructure abnormalities of retinal each layer cell were more severe than 6, 24 h. In model group, part of the cytoplasm ganglion cell organelles of ganglion cell layer dissolved, disappeared, nucleus pycnosis, heterochromatin increased, chromatin edge set. In salvia miltiorrhiza group and PC low-dose group, the organelles of ganglion cells in the cytoplasm swelling. The ganglion cell layer structure of PC high-dose group was normal. Conclusion PC has an inhibitory effect on NF-κB expression, so as to has protective effect from RIR damage.关键词
缺血再灌注损伤/原花青素/核转录因子-κB/视网膜/超微结构/大鼠Key words
ischemia reperfusion injury/ proanthocyanidins/ NF-KB/ retina/ ultrastructure/ rats分类
医药卫生引用本文复制引用
刘莹,张花治,白丽君,颉瑞萍..原花青素对视网膜缺血再灌注损伤大鼠视网膜结构及核转录因子-κB 表达的影响[J].中国中医药信息杂志,2013,20(5):32-34,3.基金项目
甘肃省自然科学研究基金(1010RJZA137) （1010RJZA137）
