中国中医药信息杂志2013，Vol.20Issue(5)：42-44,3.DOI:10.3969/j.issn.1005-5304.2013.05.015
黄芪甲苷对体外培养胚胎神经干细胞增殖作用的影响
Effect of Astragaloside on Proliferation of Neural Stem Cells Cultured in Vitro
摘要
Abstract
Objective To investigate the effect of astragaloside on the proliferation of neural stem cells (NSCs) in vitro. Methods The primitive NSCs of embryonic day 16 SD rat were cultured in vito. The NSCs were identified by immunocytochemical (ICC) staining of Nestin. The ICC staining of BrdU was adopted to characterize proliferation of NSCs. The effect of the different dosage astragaloside groups (6, 0.6, 0.06 μmol/L) on proliferation of NSCs was valued by limited dilution method. Results The NSCs were successfully cultured in vitro. Characteristic protein (Nestin) of NSCs labeling and BrdU labeling were positive. Compared with the control group, the numbers of neruospheres of the different dosage astragaloside groups were increased obviously. Conclusion Astragaloside can significantly increase the number of neruospheres and promote the proliferation of NSCs in vitro.关键词
神经干细胞/黄芪甲苷/增殖Key words
neural stem cells/ astragaloside/ proliferation分类
医药卫生引用本文复制引用
钟佩茹,王秀云,柴丽娟,周志焕,石田寅夫..黄芪甲苷对体外培养胚胎神经干细胞增殖作用的影响[J].中国中医药信息杂志,2013,20(5):42-44,3.基金项目
2005 年度日本国文部科学省补助金援助资助项目(16 文科高第978 号) （16 文科高第978 号）
