重庆医学2013，Vol.42Issue(15)：1701-1702,1705,3.DOI:10.3969/j.issn.1671-8348.2013.15.007
miRNA-142-5p在特应性皮炎中的表达及其靶基因预测
Expression of miRNA-142-5P in atopic dermatitis and its target gene prediction
摘要
Abstract
Objective To detect the expression levels of miRNA-142-5p in peripheral blood mononuclear cell (PBMC) from the patients with atopic dermatitis (AD) and to predict its latent target genes .Methods 8 cases of AD treated in the dermatological outpatient department of this hospital from October 2011 to February 2012 were selected as the observation group and 6 age-matched and sex-matched individuals with physical examination as the control group .PBMC were isolated .miRNAs in PBMC were extracted and separated by miRNA extraction and separation kit .The expression levels of miRNA-142-5p in PBMC were detected by using real-time quantitative PCR .Six databases including miRanda ,PicTar and Targetscan were adopted for predicting the targets of selected miRNAs .Results Compared with the control group ,the relative expression levels of miRNA-142-5p in PBMC of AD patients were up-regulated (P<0 .05) .A total of 39 target genes for miRNA-142-5p were shared between these five databases , which might be involved in signal transduction ,apoptotic process and cell differentiation .Conclusion miRNA-142-5p is up-regulated in the PBMC of AD patients ,which may play an important role in the pathogenesis of AD by regulating the downstream target gene.关键词
微RNAs/皮炎,特应性/聚合酶链反应/靶基因修复/预测Key words
microRNAs /dermatitis ,atopic /polymerase chain reaction /targeted gene repair forecasting引用本文复制引用
肖能鑫,史丙俊,刁庆春,王修勇,蒋有让,闫国富..miRNA-142-5p在特应性皮炎中的表达及其靶基因预测[J].重庆医学,2013,42(15):1701-1702,1705,3.基金项目
重庆市自然科学基金资助项目(cstc2012jjA10141). （cstc2012jjA10141）
