感染、炎症、修复2013，Vol.14Issue(1)：11-14,4.DOI:10.3969/j.issn.1672-8521.2013.01.003
二十碳五烯酸对热打击后肠上皮细胞的影响
Effects of eicosapentaenoic acid on permibility change in intestinal epithelial barrier function induced by heat-stress
摘要
Abstract
Objective:To discuss the effect of eicosapentaenoic acid (EPA) on tight junction (TJ) dysfunction of intestinal epithelium after heat stress. Methods: Human intestinal epithelial Caco-2 cells were pre-incubated with various concentration of EPA (25,50,100,150 μmol/L) for 96 hours, and then they were exposed to a temperature of 43 ℃ for 1 hour. Cell counting kit-8 (CCK-8) assay was used to detect the cellular proliferation. The barrier integrity of TJ was measured by detecting transepithelial electrical resistance (TEER) and horseradish peroxi-dase (HRP) permeability with transwell. Level of TJ protein occludin was assayed by Western blot. Change in cy-toskeleton was observed by microscopy after Coomassie blue staining. Results: EPA in 50 μmol/L concentration showed the most effective effects on promoting cell proliferation, and a decrease in TEER and impairment of intestinal permeability induced by heat exposure (all P <0. 01) were attenuated as compared to the other concentration groups. The function of occludin expression was most significantly elevated by 50 μmol/L EPA. The distortion of cytoskeleton was also effectively prevented by pretreatment with 50 μmol/L EPA. Conclusions: This study indicates that 50 μmol/L EPA is potent in protecting intestinal epithelial barrier function against heat-induced permeability dysfunction and damage of tight junction.关键词
中暑/二十碳五烯酸/紧密连接/肠黏膜屏障Key words
Heat stress/Eicosapentaenoic acid/ Tight junction/Intestinal barrier引用本文复制引用
肖桂珍,袁芳芳,古正涛,刘志锋,张亚历,苏磊..二十碳五烯酸对热打击后肠上皮细胞的影响[J].感染、炎症、修复,2013,14(1):11-14,4.基金项目
国家自然科学基金资助项目(81071529) （81071529）
