感染、炎症、修复2013，Vol.14Issue(1)：15-18,4.DOI:10.3969/j.issn.1672-8521.2013.01.004
PNU282987对烧伤休克犬复苏时脂质过氧化损伤和组织含水率的影响
Effect of PNU282987 on lipid peroxidative injury and tissue water content in dogs with lethal burn shock
摘要
Abstract
Objective: To investigate the effect of PNU282987 , a cholinergic agonist, on lipid peroxidative injury and tissue water content in dogs with lethal burn shock. Methods: Twelve adult male Beagle dogs were subjected to 50% total body surface area (TBSA) full-thickness flame injury, and then they were randomly divided into two groups, resuscitation group and PNU282987 group, with 6 animals in each group. The dogs in resuscitation group received Ringer' s solution, and PNU282987 group received equal amount of Ringer' s solution containing PNU282987 (0. 38 mg/kg, diluted in Ringer' s solution) 0. 5 hour after injury. The delivery rate and volume of Ringer's solution was in accordance with that of Parkland formula(4 ml · kg-1· 1 % TBSA-1): in the first 8 hours half of the total volume was transfused uniformly, in the later 16 hours the other half of the total volume was transfused uniformly. The plasma levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were continuously determined before and 2, 4, 8, 12 and 24 hours after burn. The dogs were sacrificed at 24 hours after burn, and heart, liver, spleen, lung, kidney and intestine were harvested for assessment of tissue water content (ratio of dry to wet weight). Results: The SOD activity was significantly decreased after burn compared to that before injury in both groups. The activity of SOD in PNU282987 group was significantly higher than that of the resuscitation group from 4 hours on after burn(P<0. 05), while the level of SOD in resuscitation group decreased progressively. The plasma levels of MDA increased in both two groups after burn, the level of MDA in PNU282987 group was significantly lower than that of the resuscitation group from 4 hours on after burn(P<0. 05). The tissue water contents were significantly lower in PNU282987 group than resuscitation group in heart[(68. 6 ± 1. 1) % vs. (78.3 ± 1.8)%], liver[(70. 0 ± 1.4)% vs. (79. 8 ± 0. 7) %] , spleen[(67. 2 ± 1. 2)% vs. (78. 8 ±0. 8) %] , lung [(74.3 ± 0. 5)% vs. ( 80.2 ± 1. 6)%], kidney[(71. 2 ± 0. 8)% vs. ( 80. 1 ± 0. 9)%] and ileum [(68. 9 ± 1. 1)% vs. (78. 7 ± 0. 8) %] respectively, all P<0. 05. Conclusions: The results indicate that PNU282987 can alleviate visceral tissue edema and lower the lipid peroxidative injury in dogs with lethal burn injury.关键词
烧伤/休克/胆碱能激动剂/氧自由基/水肿Key words
Burns/Shock/Cholinergic agonists/Oxygen free radical/Edema引用本文复制引用
杜明华,罗红敏,王欢,林志龙,马丽,胡森..PNU282987对烧伤休克犬复苏时脂质过氧化损伤和组织含水率的影响[J].感染、炎症、修复,2013,14(1):15-18,4.基金项目
国家973项目(2012CB 518101) （2012CB 518101）
