江西农业学报2013，Vol.25Issue(3)：88-92,5.
ETEC K99、K88菌毛蛋白抗原表位多肽的设计合成及免疫反应
Design Synthesis and Immunological Reaction of Epitope Peptides of ETEC K88 and K99 Fimbrial Protein
摘要
Abstract
In order to investigate the epitopes of ETEC K88 and K99 fimbrial protein, we designed and synthesized the epitope peptides of ETEC K88 and K99 fimbrial protein by bioinformatical and structural analysis software. The epitope peptides of ETEC K88 and K99 fimbrial protein were designed and synthesized after predicting and analyzing the relational parameters of the antigeni-city of ETEC K88 and K99 fimbrial protein with bioinformatics, DNAStar Protean and online sharing software. C57BL/6 mice were immunized with these synthetic peptides, the antibodies in serum were measured by ELJSA, and the challenge test was implemented on the immune mice. The results showed that two synthetic peptides could induce high titers of specific antibodies after immunological reaction. The results of challenge test with C83912 or C83902 showed that protection activity was found in the mice immunized with these synthetic peptides. It was concluded that the epitopes were successfully predicted, and the synthetic epitope peptides showed high antigenicity.关键词
ETEC K99菌毛蛋白/ETEC K88菌毛蛋白/抗原表位/合成肽Key words
ETEC K99 fimbrial protein/ ETEC K88 fimbrial protein/ Epitope/ Synthetic peptide分类
农业科技引用本文复制引用
余丽芸,田斌,温丽娟,王桂华,曹宁,魏小曼,郭东伟,齐浩,刘秋晨..ETEC K99、K88菌毛蛋白抗原表位多肽的设计合成及免疫反应[J].江西农业学报,2013,25(3):88-92,5.基金项目
黑龙江省教育厅科学技术研究项目(12511366). （12511366）
