临床与实验病理学杂志2013，Vol.29Issue(5)：472-476,5.
乳腺癌干细胞亚群中MMP-9和BRMS1的表达及其临床病理意义
Significance of MMP9 and BRMS1 in metastasis and invasion of breast cancer stem cell
摘要
Abstract
Purpose To investigate the significance of metastasis and invasion of MMMP-9 and BRMS1 in breast cancer stem cell. Methods The CD44 + CD24+ and non CD44 + CD24+ cells from MCF-7 was separated by immunomagnetic beads. The level of ALDH1 mRNA was measured by RT-PCR in different groups. The ability of metastasis and invasion in different groups cells was detected by Tr-answell. 65 patients with breast cancer tissues were divided into two groups, one is breast cancer with lymph nodes metastasis, and another is not. MMP9, BRMS1 and ALDH1 were detected in different groups by SP immunnohistochemical staining. Result The level of ALDH1 mRNA in CD44 + CD24- group was higher than other groups. The capability of immigration and invasion in CD44 + CD24-group was higher than that in other two groups ( P <0. 05 ). Western blot demonstrated that the level of MMP9 and ALDH1 in CD44 + CD24+ group was higher than non CD44+ CD24+ cells, but BRMS1 level in CD44+ CD24+ group was lower than that in non CD44 + CD24- cells ( P<0. 05 ). The expression of MMP9 and ALDH1 in breast cancer tissues with lymph node metastasis was higher than that without lymph node metastasis. However,the expression of BRMS1 in breast cancer with lymph node metastasis was lower than that without lymph node metastasis. The expression of ALDH1 was positively correlated with that of MMP-9 and negatively with that of BRMS1 ( P <0. 05 ). Conclusion MMP-9 and BRMS1 may play an important role in the metastasis and invasion of breast cancer stem cell.关键词
乳腺癌干细胞/MMP-9/BRMS1/ALDH1/转移Key words
breast cancer stem cell/MMP-9/BRMS1 /ALDH1/metastasis分类
医药卫生引用本文复制引用
王丽霞,毛俊,陶雅军,马威,张晴晴,王波,范姝君,李连宏..乳腺癌干细胞亚群中MMP-9和BRMS1的表达及其临床病理意义[J].临床与实验病理学杂志,2013,29(5):472-476,5.基金项目
国家自然科学基金(81272430),教育部高等学校博士学科点专项科研基金(200801610001) （81272430）
