临床与实验病理学杂志2013，Vol.29Issue(5)：511-516,6.
癌相关成纤维细胞对结肠癌细胞增殖侵袭能力的影响
Effect of colon cancer associated fibroblasts on the proliferation and invasion of colon cancer cell line
摘要
Abstract
Purpose To investigate the effect of purified culture of the colon cancer associated fibroblast ( CAF ), normal fibroblast ( NF ) on the proliferation and invasion of colon cancer cell line, and to study the the role of cancer cell' s micro-environment in the development of colorectal cancer. Methods CAF from colon carcinoma samples and normal colon fibroblast were collected to primary and pure cultivate. The morphology, proliferating and invasive of HCT-116 cell lines treated with CAF and fibroblast were observed. Results Purified cells of colon CAF were more dysplasia, loss of the contact and density inhibition was seen. CAF and NF cells were negative with cytokeratin CK( AE1/AE3 ) and positive with fibronectin, CAF was positive fora-SMA, but NF was negative. MTT results showed the proliferative activity of HCT-116 by adding the conditioned medium of CAF was higher than NF conditioned medium or the serum-free medium ( P <0. 05 ). Flow cytometry analysis showed that G2 and S phase cell percentage in HCT-116 co-cultured with CAF obviously increased . Under the action of CAF, the invasion ability of HCT-116 cells significantly enhanced ( P <0. 05 ). Conclusions Colon CAF may promote the proliferation and invasion of colon cancer cell lines HCT-116. CAF conditioned medium may contain some soluble substances which can promote the proliferation and invasion of HCT-116 cells.关键词
结肠肿瘤/癌相关成纤维细胞/微环境/增殖/侵袭Key words
colon neoplasms/cancer associated fibroblast/microenvironment/proliferation/invasion分类
医药卫生引用本文复制引用
王甜甜,陆建波,普苹,王春艳,习杨彦彬,付红梅..癌相关成纤维细胞对结肠癌细胞增殖侵袭能力的影响[J].临床与实验病理学杂志,2013,29(5):511-516,6.基金项目
云南省科技厅昆明医学院应用基础研究联合基金(2008CD004R) （2008CD004R）
