农业生物技术学报2013，Vol.21Issue(3)：355-366,12.DOI:10.3969/j.issn.1674-7968.2013.03.013
染色体步移技术克隆已知序列侧翼启动子的研究进展
Progress of Chromosome Walking the Unknown Promoter Sequences Flanked by a Known Segment
摘要
Abstract
Promoter is a key cis-acting element to regulate gene expression, which is also an important element of the expression vector in genetic engineering. Understanding the structure and function of promoters contributes to constructing vector and controlling the gene expression, further we can achieve the goal that induces protein expression selectively. Therefore introducing a proper method to clone the promoter presents significance and wide application prospects. Since the invention of polymerase chain reaction (PCR) technique, several PCR-based chromosome walking methods have been developed to amplify unknown promoter sequences flanked by a known segment. In this review, we introduce the PCR-based methodologies for the rapid acquisition of unknown promoter, including Inverse PCR, Ligation-mediated PCR, Semi-random primer PCR. These principles and research progresses of each approach are discussed.关键词
染色体步移/启动子/反向PCR/接头介导PCR/半随机引物PCRKey words
Chromosome walking/ Promoter/ Inverse PCR/ Ligation-mediated PCR/ Semi-random primer PCR引用本文复制引用
康丹,方小艳,游腾飞,王婷婷,眭安平,杨星勇..染色体步移技术克隆已知序列侧翼启动子的研究进展[J].农业生物技术学报,2013,21(3):355-366,12.基金项目
国家自然科学基金项目(No.30771388) （No.30771388）
