山西医科大学学报2013，Vol.44Issue(5)：354-357,4.DOI:10.3969/J.ISSN.1007-6611.2013.05.008
大黄素抑制SHG44胶质瘤细胞增殖及诱导凋亡的研究
Effect of emodin on cell proliferation and apoptosis of human glioma cells SHG44
摘要
Abstract
Objective To explore the effects of emodin on proliferation and apoptosis of human glioma cells SHG 44.Methods The cultured SHG44 cells were treated with 10,20,40 and 80 μmol/L emodin in experimental groups,respectively,but not treated in control group The inhibitive effect of emodin on SHG 44 cell proliferation was detected by MTT assay , cell apoptosis was detected by flow cy- tometiy ,and the relative activity of caspase-3 was detected by colorimetry.Results Compared with control group , emodin significantly inhibited the proliferation of SHO44 cells in a dose-dependent and time-dependent manner ,and the IC50(48 h) of emodin was 24.79 μmol/L.Flow cytometry analysis showed that emodin dose -dependently induced the apoptosis in SHO44 cells after 12 h treatment(P < 0.05 ).The relative activity of caspase-3 was elevated timedependently after emodin treatment ( P < 0.05 ).Conclusion Emodin can inhibit the proliferation of glioma cells SHG44 and induce the apoptosis in SHG44 cells,which may be related to the caspase-3 activation.关键词
大黄素/胶质瘤/细胞增殖/细胞凋亡Key words
emodin/ glioma/ cell proliferation/ cell apoptosis分类
医药卫生引用本文复制引用
张鹏,许刚柱,刘凯歌,张军伟,王茂德..大黄素抑制SHG44胶质瘤细胞增殖及诱导凋亡的研究[J].山西医科大学学报,2013,44(5):354-357,4.基金项目
陕西省教育厅科研基金资助项目(11JK-0712) （11JK-0712）
西安医学院校级科研基金资助项目(11FZ25) （11FZ25）
西安医学院附属医院科研基金资助项目(XYFY10-13) （XYFY10-13）
