山西医科大学学报2013，Vol.44Issue(5)：374-376,3.DOI:10.3969/J.ISSN.1007-6611.2013.05.014
抗结核药PAS新衍生物的化学表征及其与牛血清蛋白的相互作用
Characterization of the new p-aminosalicylic acid derivative and its interaction with bovine serum albumin
摘要
Abstract
Objective To investigate the characterization of p-aminosalicylic acid ( PAS) -Schiff as a new antimyobacterial drag and its interaction with bovine serum albumin ( BSA).Methods Schiff base was prepared with salicylaldehyde and 4-aminosalicylic acid as materials.The complex was measured by elemental analysis ,IR and 1H-NMR.The interaction between Schiff base and bovine serum al-bumin(BSA) was studied by fluorescence spectra.Results Schiff base was successfully obtained.The combination of BSA with the complex was a static quenching procedure .The conditional stability constant was 2.72×105 ,2.08×105 ,7.55×104 mol/L at 15℃,25 ℃ and 35 ℃ , respectively, and the number of binding site was one at 15℃ ,25℃ and 35℃.Meanwhile, the interaction was mainly driven by hydrophobic force between Schiff base and BSA .Conclusion The results suggest that a new PAS -Schiff base is obtained and the binding force between Schiff base and BSA is hydrophobic .The binding model could be demonstrated by the binding parameters of schiff base and BSA.关键词
PAS/Schiff碱/牛血清白蛋白/荧光光谱Key words
p-aminosalicylic acid / Schiff base / bovine serum albumin/ fluorescence spectra分类
医药卫生引用本文复制引用
刘文,孙体健,王浩江,弓辉,刁海鹏..抗结核药PAS新衍生物的化学表征及其与牛血清蛋白的相互作用[J].山西医科大学学报,2013,44(5):374-376,3.基金项目
山西省高校高校高新技术产业化基金资助项目(20120015),山西省高等学校大学生创新创业训练基金资助项目(2012116),山西医科大学科技创新基金资助项目(C01201003) （20120015）
