武警医学2013，Vol.24Issue(5)：383-386,4.
血清MicroRNA-122作为脓毒症诊断特异性标志物的研究
Serum microRNA-122 as a specific marker of sepsis diagnosis
摘要
Abstract
Objective To study the dynamic changes in serum miRNA - 122 levels during sepsis and the correlation between serum miRNA - 122 and sepsis severity. Methods Fifty - two sepsis patients (including 23 non - severe septic patients and 29 severe septic patients) and 23 healthy controls were recruited in the study. The expression levels of serum miRNA - 122 were quantified by quantitative reverse transcriptase polymerase chain reaction assays ( qRT - PCR). Levels of serum miRNA - 122 were detected on the 1 st, 3 rd, 5 th, 7 th, 10 th and 14 th day after their admissions in intensive care unit. All the patients'clinical information was also recorded. Results After comparison, levels of serum miRNA - 122 in sepsis patients on every observation time point were all significantly lower than those in healthy controls ( P < 0. 01 ) . The receiver operating characteristic curve was obtained and the area under curve was calculated on the levels of serum miRNA - 122 on the first day of their admissions. When the cut - off point was set at 0. 710, miRNA - 122 had a specificity of 95. 7% and a sensitivity of 53. 8% . The expression levels of serum miRNA - 122 in severe sepsis group were significantly higher than in sepsis group on each observation time point ( P < 0. 01) . Conclusions Serum miRNA -122 can used as a specific biomarker for diagnosis of sepsis. And miRNA - 122 might be correlated with the organ injuries of septic patients.关键词
脓毒症/微小RNA-122/微小RNA/诊断Key words
sepsis/ microRNA - 122/ microRNA/ diagnosis分类
医药卫生引用本文复制引用
邓婕,王慧娟,苏龙翔,张鑫,肖坤,贾艳红,解立新..血清MicroRNA-122作为脓毒症诊断特异性标志物的研究[J].武警医学,2013,24(5):383-386,4.基金项目
国家自然科学基金(81170008) （81170008）
军队十二五面上课题(CWS11J094) （CWS11J094）
