中国人兽共患病学报2013，Vol.29Issue(5)：433-441,9.DOI:10.3969/cjz.j.issn.1002-2694.2013.05.003
感染日本血吸虫的湖北钉螺三种血淋巴细胞分布的组织学观察
Histological observations on the distribution of three types of haemo-lymphocytes in Oncomelania hupensis (Gastropoda: Pomatiopsidae) infected with Schistosoma japonicum
摘要
Abstract
Knowledge of defense mechanism of O. hupensis, particularly their haemo-lymphocytes, which are considered to be the main effectors of invertebrate defense system, should be beneficial to control the disease transmission. In this study, qualitative and quantitative investigations of haemo-lymphocytes in negative and positive O. hupensis after infection with S. japonicum was conducted by histological sectioning of infected snails at post-infection days 3, 5, 7, 12, 16, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, and 90. H & E stained haemo-lymphocytes had an appearance with circular nucleus and densely packed granules inside the cell with entire cytoplasm stained deep blue in color. The haemo-lymphocytes were divided into three types based on their nucleus sizes: large, medium, and small, with diameter ranges of 8.1-8.6 μm (large), 5.5-6.0 μm (medium), and 4.2-4.7 μm (small). Post-infection increase in numbers of haemo-lymphocytes in five organs of infected snails including foot, pericardial, gastrointestinal, alimentary gland, and genital organs regions was observed. The rich area of haemo-lymphocytes was in the pericardial region. Moreover, the fewest number of haemo-lymphocytes presented in foot region. The numbers of haemo-lymphocytes with three nucleus sizes in all snail body parts were clearly increased at beginning, then slightly decreased later, and the haemo-lymphocytes with medium nucleus size was the dominated population. The haemo-lymphocytes with large and small nucleus sizes were found but few in number, Data gathered during the present study should be useful in understanding the defense mechanism of O. hupensis in response to S. japonicum infection, and further the research with the aim of controlling the disease more effectively.关键词
胃肠炎/爆发/诺如病毒/基因型Key words
Schistosoma japonicum/ Oncomelania hupensis/ haemo-lymphocytes/ distribution分类
医药卫生引用本文复制引用
Theerakamol Pengsakul,Yassir A Suleiman..感染日本血吸虫的湖北钉螺三种血淋巴细胞分布的组织学观察[J].中国人兽共患病学报,2013,29(5):433-441,9.基金项目
Supported by the Natural Science Foundation of People's Republic of China (No.31270938) （No.31270938）
