中国人兽共患病学报2013，Vol.29Issue(5)：456-459,4.DOI:10.3969/cjz.j.issn.1002-2694.2013.05.007
原位末端标记法观察钉螺细胞凋亡的研究
Observation on apoptosis in Oncomelania hupensis by TUNEL technique
摘要
Abstract
Thirty snails were divided into two groups for exploring the potential benefit of terminal deoxynucleotide transferasemediated dUTP-biotin nick end labeling assay (TUNED in research pertaining to Oncomelania hupensis by observing its effect in cellar apoptosis. Twenty snails in experimental group were immersed in the leaf extracts of Melia azedarac for 24 hours and soft tissues were separated for frozen section (6 μm). The cellar apoptosis of snails were observed by TUNEL technique and hematoxylin and eosin (HE) staining. Others served as control group. Results showed that there were clusters of positive signal in both experimental and control groups. Apoptotic cells were detected in digestive gland, foot tegument and muscle of experimental snails and in digestive gland and foot tegument of control group. It was found that apoptosis index of experimental group was significantly higher than that in the control (P<0. 05). There was no significant difference in apoptosis index between TUNEL technique and HE staining (P>0. 05). So we concluded that apoptotic cells and apoptosis index in O. hupensis can be detected using TUNEL technique, which indicates that the technique holds promise in relevant studies.关键词
钉螺/细胞凋亡/原位末端标记法Key words
Oncomelania hupensis / apoptosis/ TUNEL分类
医药卫生引用本文复制引用
顾文彪,张仪,刘和香,吴缨,朱丹,吕山,夏尚光,王龙杰..原位末端标记法观察钉螺细胞凋亡的研究[J].中国人兽共患病学报,2013,29(5):456-459,4.基金项目
国家"十二五"林业生态科技项目 (2011BAD38B070101)资助 （2011BAD38B070101）
