中国人兽共患病学报2013，Vol.29Issue(5)：472-475,4.DOI:10.3969/cjz.j.issn.1002-2694.2013.05.010
刚地弓形虫苹果酸脱氢酶基因编码蛋白主要特性与抗原表位的生物信息学分析
Bioinformatics analysis of main characteristics and epitopes of the gene encoding malate dehydrogenase protein from Toxoplasma gondii
摘要
Abstract
The immunogenicity of the malate dehydrogenase protein extracted from Toxoplasma gondii (TgMDH) was predicted and its structure and epitopes were characterized by bioinformatics approaches. The open reading frame, physico-chemical properties, solublity, signal peptide, cross membrane area, surface accessibility, flexibility, hydrophilicity, post-translational modification site, secondary structure, and antigen epitope of TgMDH protein were analyzed and predicted by bioinformatics online analysis programs which were provided by expert protein analysis system (ExPASy) including Prot-Param, SOSUI, TMPRED, HNN, MotifScan, ORF finder, SignalP and Bcepred, combining with bioinformatics softwares (Gene Runner and DNAMAN). The three dimensional structure of the protein was then processed by CPHmodels. Results indicated that TgMDH protein was composed of 316 amino acids with listed characters: the molecular formula was C149852454 N402 O440S20, the molecular mass was 33 777. 5, the value of theoretical isoelectric point was 6. 01, the absorbance (A280) value was 0. 364 , and the half-life period was 30 h. There were nine zones with surface accessibility≥1. 9 , four zones with hydrophilicity ≥1. 9, seven zones with flexibility≥2, fourteen post-translational modification sites and fourteen potential epitopes as soluble protein expression. It's suggested that TgMDH protein could be used as a candidate antigen for toxoplasmosis vaccination because of its soluble protein expression and immunogenic property.关键词
刚地弓形虫/苹果酸脱氢酶/生物信息学/抗原表位/免疫原性Key words
Toxoplasma gondii/ malate dehydrogenase/ bioinformatics/ epitope/ immunogenicity分类
医药卫生引用本文复制引用
刘转转,杨燕萍,郑葵阳,刘宜升..刚地弓形虫苹果酸脱氢酶基因编码蛋白主要特性与抗原表位的生物信息学分析[J].中国人兽共患病学报,2013,29(5):472-475,4.基金项目
江苏省寄生虫病预防与控制高技术平台开放课题(No.WK009-003) （No.WK009-003）
徐州医学院科研课题(No.2012KJ08)联合资助 （No.2012KJ08）
