中国人兽共患病学报2013，Vol.29Issue(5)：486-489,4.DOI:10.3969/cjz.j.issn.1002-2694.2013.05.013
弓形虫ROP5基因与基因佐剂IL-12对小鼠免疫保护性
Protective efficacy induced by DNA vaccine encoding ROP5 co-immunized with IL-12 genetic adjuvant against Toxoplasma gondii infection
摘要
Abstract
The purpose of this study was to examine the immunoprotection effect induced by ROP5 DNA vaccine with co-immunization of a plasmid encoding IL-12 (pcmIL-12) as an adjuvant in Kunming mice against Toxoplasma gondii. Mice were co-injected ROP5 with IL-12 at the dosage of 100 μg, controls mice were inoculated with PBS and the empty plasmid pcD-NA3. 1 respectively. Levels of IgG antibody, IgG isotype, gamma-interferon (IFN-γ), and interleukin-4 were detected by ELISA. MTT assay was used to tested splenocyte proliferation. All mice were challenged with tachyzoites of the virulent T. gondii RH strain after the last immunization to observe the survival time. In this study we detected the higher level of IgG antibody and cytokine IFN-γ in mice after three immunizations by pcROP5 co-immunized with pcmIL-12 compared to pcROP5 group and control groups. In respect to the IgG isotypes, co-immunization with pcmIL-2 enhanced the level of IgG2a, while IgG1 and IL-4 level was not appreciably different. After a lethal challenge of T. gondii RH strain, survival time of the mice co-immunized with pcmIL-12 was prolonged compared to the control groups. Immune responses could be enhanced by ROP5 co-immunized with IL-12 genetic adjuvant.关键词
DNA疫苗/弓形虫抗原ROP5/基因佐剂pcmIL-12/免疫应答Key words
DNA immunization/ Toxoplasma gondii antigenic ROP5/ gene adjuvant IL-12/ immunological response分类
医药卫生引用本文复制引用
赵焕阁,林映莹,黄用豪,黄风迎,周松林,刘中娟,谭光宏,韦? ..弓形虫ROP5基因与基因佐剂IL-12对小鼠免疫保护性[J].中国人兽共患病学报,2013,29(5):486-489,4.基金项目
海南省自然科学基金(No.809018)资助 （No.809018）
