中国人兽共患病学报2013，Vol.29Issue(5)：516-519,4.DOI:10.3969/cjz.j.issn.1002-2694.2013.05.020
青岛市售养殖海水虾中副溶血性弧菌的分离及耐药性分析
Isolation and antimicrobial resistance of Vibrio parahaemolyticus in farmed marine shrimps from Qingdao markets
摘要
Abstract
The Vibrio parahaemolylicus strains in farmed marine shrimps from Qingdao markets were isolated and identified and the antimicrobial resistance of these isolates was tested. Presumed V. parahaemolylicus in shrimps were isolated by traditional culture method and identified by automatic bacterial identification system. The virulence genes were determined by PCR amplification and the antimicrobial resistance was performed by K-B method. A total of 50 (78. 1%) V. parahaemolylicus strains were isolated from 64 shrimp samples. The tdh gene was absent in all the isolates and trh gene was found in three of them. All isolates were resistant to cephradine and 96% of them were resistant to ampicillin and amoxycillin. Some isolates were resistant to cefuroxime sodium, streptomycin, tetracycline, oxytetracycline, and sulphamethoxazole/trimethoprim. A few isolates exhibited multidrug-resistance against at least three kinds of antibiotics. The results indicated that the farmed marine shrimps from Qingdao markets are highly contaminated by V. parahaemolylicus and the isolates have antimicrobial resistance to a certain extent.关键词
海水虾/副溶血性弧菌/分离鉴定/毒力基因/耐药性Key words
marine shrimp/ V. parahaemolylicus/ isolation and identification/ virulence gene/ antimicrobial resistance分类
医药卫生引用本文复制引用
江艳华,姚琳,李风铃,王联珠,宋春丽,翟毓秀..青岛市售养殖海水虾中副溶血性弧菌的分离及耐药性分析[J].中国人兽共患病学报,2013,29(5):516-519,4.基金项目
黄海水产研究所级基本科研业务费(20603022012010)资助 （20603022012010）
