中国医学科学院学报2013，Vol.35Issue(2)：155-160,6.DOI:10.3881/j.issn.1000-503X.2013.02.006
大肠癌患者化疗前后淋巴细胞亚群变化
Changes of Lymphocyte Subsets before and after Chemotherapy in Colorectal Carcinoma Patients
摘要
Abstract
Objective To investigate the change of lymphocyte subsets before and after chemotherapy in colorectal carcinoma patients. Methods Twenty-one peripheral blood lymphocyte subsets from 62 colorectal carcinoma patients before and after F0LF0X4 (including oxaliplatin, 5 -fluorouracil and leucovorin) , FOLFRI (including irinotecan, 5 -fluorouracil and leucovorin ) , or XELOX ( including oxaliplatin and capecitabine ) regimen chemotherapy were examined by flow cytometry. The differences of these lymphocyte subsets were ana-lyzed. Results After chemotherapy, the percentages of CD3 + , CD3 + CD8 + , CD29 + , CD4 + CD29 + , and CD4 + CD25 + cells in peripheral blood of colorectal carcinoma patients increased significantly, while the percentages of CD19+ and human leukocyte antigen (locus) DR (HLA-DR) + cells decreased significantly (P < 0. 05 ). The results of subgroup analysis showed that the patients' CD3 + CD8 + and CD4 + CD25 + cells increased significantly, CD19+ and HLA-DR+ cells decreased significantly after FOLFOX4 regimen chemotherapy (P< 0. 05) ; CD3 + CD8 + cells increased significantly and CD19+ cells decreased significantly after XELOX regimen chemotherapy (P < 0. 05 ) ; while after FOLFRI regimen chemotherapy, there were no significant changes in all 21 lymphocyte subsets ( P > 0. 05 ) . CD3 + , CD3 + CD8 + , memory T lymphoctye ( 45RO + ) , and CD4 + CD45RO+ cells increased significantly (P<0. 05) in patients who received no more than 4 cycles of chemotherapy. However, in patients that received 5 to 8 cycles and more than 9 cycles chemotherapy, we only found significant decrease of HLADR + cells and significant increase of CD29 + cells, respectively (P < 0. 05). Conclusions The humoral immunity is attenuated after chemotherapy in colorectal carcinoma patients. FOLFOX4 may suppress the cellular immunity. Chemotherapy that is less than 4 cycles will strengthens the cellular immunity by modulating body immunity arrangement; however, along with the increase of chemotherapy cycles, the cellular immunity gradually declines in these patients.关键词
大肠癌/化疗/免疫功能/淋巴细胞亚群Key words
colorectal carcinoma/ chemotherapy/ immunity/ lymphocyte subsets分类
医药卫生引用本文复制引用
张国庆,赵宏,温新宇,李瑾昱,吴亮亮,焦顺昌..大肠癌患者化疗前后淋巴细胞亚群变化[J].中国医学科学院学报,2013,35(2):155-160,6.基金项目
国家科技部支撑计划基金(2009BAI86B05)Supported by the Foundation of National Science and Technology Infrastructure Program(2009BAI86B05) （2009BAI86B05）
第一、二位作者对本文贡献相同The first two authors contributed equally to this article （）
