中国医学科学院学报2013，Vol.35Issue(2)：171-176,6.DOI:10.3881/j.issn.1000-503X.2013.02.009
化疗后残存乳腺癌组织中乳腺癌耐药蛋白、P-糖蛋白的表达及其与癌干细胞的相关性
Expression of Breast Cancer Resistance Protein and P-glycoprotein in Residual Breast Cancer Tissue after Chemotherapy and Its Correlation with Cancer Stem Cells
屈洪波 1范原铭 1韩明利 1罗浩军 1谢佳 1刘红 1刘毫 1吴诚义 1汤为学2
作者信息
- 1. 重庆医科大学,附属第一医院内分泌乳腺外科,重庆,400016
- 2. 重庆医科大学,附属第一医院重庆市神经病学重点实验室,重庆,400016
- 折叠
摘要
Abstract
Objective To compare the expression differences of breast cancer resistance protein (BCRP/ABCG2) and P-glycoprotein (P-gp) in breast cancer tissue before chemotherapy and in residual breast cancer tissue, and to explore its correlation with breast cancer stem cells. Methods Immunohistochemistry was used to detect the expression of ABCG2, P-gp, and breast cancer stem cells (BCSCs ) markers ( CD44 and CD24 ) in breast cancer tissue before chemotherapy and residual breast cancer tissue after chemotherapy. Immu-nofluorescence was applied for determination of the CD44 and CD24 protein expressions of BCSCs microspheres cells. The monoclone-forming ability of BCSCs microspheres cells was detected by limited dilution assay. The ex-pressions of ABCG2 , P-gp, CD44 , and CD24 proteins were detected by Western blot. Results Compared with those in breast cancer tissue before chemotherapy, the expression levels of ABCG2 and P-gp were positively correlated with the expression level of CD44 protein (χ2 = 41. 34 , r = 0. 83 ; χ2 = 22. 81, r = 0. 61 ) in residual breast cancer tissue after chemotherapy; meanwhile, they were negatively correlated with the expression of CD24 protein (χ2 = - 21. 25 , r = 0. 72; χ2 = - 17. 26 , r = 0. 65 ) ( all P < 0. 05 ) . The diameter of BCSCs micro-spheres were increased significantly after chemotherapy. The content of BCSCs increased by about 2. 5 times after chemotherapy. The expressions of ABCG2, P-gp and CD44 proteins significantly increased and that of CD24 protein significantly declined (P < 0. 05 ) . Conclusion Chemotherapy endows residual breast cancer tissue with cancer stem cells-like feastures, leading to multidrug resistance of breast cancer.关键词
乳腺癌干细胞/耐药相关蛋白/乳腺癌耐药蛋白/P-糖蛋白/化疗抵抗性Key words
breast cancer stem cells/ resistance-associated protein/ breast cancer resistance protein/ P-glycoprotein/ chemotherapy resistance分类
医药卫生引用本文复制引用
屈洪波,范原铭,韩明利,罗浩军,谢佳,刘红,刘毫,吴诚义,汤为学..化疗后残存乳腺癌组织中乳腺癌耐药蛋白、P-糖蛋白的表达及其与癌干细胞的相关性[J].中国医学科学院学报,2013,35(2):171-176,6.
