果树学报2013，Vol.30Issue(2)：202-206,5.
杧果炭疽病菌漆酶基因lac1的克隆与序列特征分析
Cloning and sequence analysis of laccase gene lac1 from Colletotrichum gloeosporioides on mango
摘要
Abstract
[Objective ]The objective of this study was to reveal sequence features of Iaccase(lac1), which would be used in further study of pathogenesis of C. gloeosporioides on Mango. [Method]A laccase gene homolog lac1 was cloned from C. gloeosporioides on mango by the method of homologous cloning, SEFA-PCR and RT-PCR, and sequence characterization and the conserved domains on predicted protein of lac1 gene were also analyzed. [Result]The results showed that the complete DNA and cDNA sequence of lac1 gene were 2 996 bp and 1 773 bp in size, respectively, with three introns of 47 bp, 50 bp and 59 bp, respectively, encoding a putative protein of 590-amino acid with a molecular weight of 1174.50 kDa and isoelectric point of 5.36. Phylogenetic analysis indicated that putative protein of lac1 was homologous to C. lagenarium laccase (BAB32575.1) with a similarity of 73%,and was also homologous to copper-containing oxidases in some other fungi at some similarities. [ Conclusion ]The studies suggested that lacl gene had the sequence characteristics of laccase gene family in fungi,which meant that LAC1 might involve in mycelia growth, pigment production and pathogenicity of C. gloeosporioides.关键词
杧果/胶孢炭疽菌/漆酶/克隆/序列分析Key words
Mango/ Colletotrichum gloeosporioides / Laccase/ Cloning/ Sequence analysis分类
农业科技引用本文复制引用
韦运谢,刘晓妹,张贺,张欣,漆艳香,谢艺贤,陆英,曹申文,蒲金基..杧果炭疽病菌漆酶基因lac1的克隆与序列特征分析[J].果树学报,2013,30(2):202-206,5.基金项目
公益性行业(农业)科研专项(201203092-2) （农业）
环植所基本科研业务费(2010hzsZDZX002) （2010hzsZDZX002）
