渔业科学进展2013，Vol.34Issue(1)：172-176,5.
黄芪多糖微胶囊制备及对刺参抗病力的影响
Preparation of astragalus polysaccharides microcapsule and its effects on disease resistance in sea cucumber Apostichopus japonicas
摘要
Abstract
Using astragalus polysaccharides as the core material, sodium alginate as the wall material, a spray coagulation bath method was developed for preparation of astragalus polysaccharides microcapsules, which are uniform particles at the sizes of 100~150μm and insoluble in water. Through phenol-sulfuric acid colorimetry, the drug loading of the microcapsule was measured as 108mg/g, and the embedding rate was 85. 7%. A 35-d feeding experiment was conducted on sea cucumbers to evaluate the effects of astragalus polysaccharides microcapsules on resistance against Vibrio splendidus. The basal diet was used as control. Four practical diets were supplemented with 3% blank microcapsule and 1%, 3%, and 5% astragalus polysaccha-rides microcapsule, respectively (Treatment 1~4). Each diet was randomly allocated to four groups of sea cucumber cultured in 200L fiberglass indoor tanks with circulating seawater and constant aeration. Each tank was stocked with 30 sea cucumbers (initial average weight 30. 00 ±1.00g). After 35 days of feeding with microcapsules or control diet, every group of sea cucumbers were injected into the body wall with 109 CFU/ml live V. splendidus bacterial stock solution at a dose of 0. 1 ml per sea cucumber (based on data of 10-day LD50 pre-test). Sea cucumbers in Treatment 3 and 4 showed significantly lower accumulative mortality than the control, or Treatment 1 or 2 (P<0. 05) , and no significant difference was observed between Treatment 3 and 4 (P>0. 05). Significant difference was also observed between Treatment 2 and the control (P<0. 05) , but no significant difference was observed either between the control and treatment 1, or between treatment 1 and 2 (P>0. 05). According to these results, the oral administration of astragalus polysaccharides microcapsules to A. japonicus at a dose of 3% or 5% enhances the immunity as well as increases disease resistance, and the protective rate may reach 66. 67% and 69.44%.关键词
刺参/黄芪多糖/微胶囊/抗病力Key words
Apostichopus japonicus/Astragalus polysaccharides/Microcapsule disease/Resistance分类
农业科技引用本文复制引用
许拉,樊英,李天保,于晓清,王淑娴,盖春蕾,叶海斌,刁菁..黄芪多糖微胶囊制备及对刺参抗病力的影响[J].渔业科学进展,2013,34(1):172-176,5.基金项目
山东省农业重大应用技术创新课题(鲁财农指[2010]58号)、海洋公益性行业科研专项经费项目(200905020)和山东省科技发展计划项目(2010GHY10501)共同资助 （鲁财农指[2010]58号）
