国际眼科杂志2013，Vol.13Issue(6)：1093-1095,3.DOI:10.3980/j.issn.1672-5123.2013.06.08
大鼠角膜碱烧伤模型制备方法的研究
Study on the establishment of corneal alkali chemical injury on rats
顾宏卫 1胡楠1
作者信息
- 1. 226001,中国江苏省南通市,南通大学附属医院眼科
- 折叠
摘要
Abstract
AIM:To investigate the appropriate methods to establish corneal alkali chemical injury on rats.METHODS:The rats (n = 87) were randomly divided into three groups. Corneal alkali injury was induced by placing 1 mol/L NaOH soaked filter paper on the limbus of right cornea for 20 seconds (group A, n = 34) or 40 seconds (group B, n=23), and on the central axis of the right cornea for 40 seconds (group C, n = 30) respectively. Corneal transparency, corneal ulceration, and corneal neovascularization were observed and recorded under slit-lamp biomicroscope on day 7 post-operation.RESULTS: Incidence of corneal ulceration, corneal perforation and positive rate of corneal fluorescein staining in limbal corneal injury groups (group A and B) were significantly higher than that of central corneal injury group (group C) (P< 0. 05). Incidence of corneal ulceration and corneal perforation in group B was significantly higher than group A (P<0. 05). Corneal neovascularization was observed in all three groups.CONCLUSION: Corneal alkali burns induced by 3mm diameter central cornea injury are fit for the study of corneal neovascularization, while those induced by limbus injury for 20 seconds are fit for the study on limbal stem cells deficiency.关键词
角膜/碱烧伤/动物模型/新生血管/大鼠Key words
corneal/ alkali injury/ animal models/ corneal neovascularization/ rat引用本文复制引用
顾宏卫,胡楠..大鼠角膜碱烧伤模型制备方法的研究[J].国际眼科杂志,2013,13(6):1093-1095,3.
