畜牧与兽医2013，Vol.45Issue(4)：18-21,4.
贝类折光马尔太虫二温式PCR检测方法的建立及初步应用
Development of two-temperature PCR for detection of Marteilia refringens in shellfish and its application
摘要
Abstract
According to the gene sequences of Marteilia refringens in GenBank, one pair of specific primers were designed for amplifying the specific fragments of M. refringens. The reaction parameters were optimized to develop the two-temperature PCR method for detection of M. refringens. The specific DNA fragment with the length of 266 bp was amplified from M. refringens, but not from other pathogens such as Haplosporidium sp. , Perkinsus sp. , Vibrio parahaemolyticus, Vibrio alginolpicus, and Vibrio fluvialis. The sensitivity results showed that as little as lfg DNA plasmid of M. refringens could be detected. 256 Shellfish samples from Fujian were detected by this two-temperature PCR. As a result, 12 razor clam samples were positive. It suggested that M. refringens existed in the cultivated shellfish in Fujian and this two-temperature PCR could be used as a sensitive tool to detect M. refringens in clinical samples.关键词
贝类/折光马尔太虫/二温式PCRKey words
shellfish/ Marteilia refringens/ two-temperature PCR分类
生物科学引用本文复制引用
谢丽基,谢芝勋,刘加波,庞耀珊,邓显文,谢志勤,范晴..贝类折光马尔太虫二温式PCR检测方法的建立及初步应用[J].畜牧与兽医,2013,45(4):18-21,4.基金项目
国家百千万人才工程人选专项(945200603)、广西特聘专家专项(2011B020)和广西科技攻关(桂科攻0630001-3M)共同资助. （945200603）
