中国全科医学2013，Vol.16Issue(15)：1740-1743,4.DOI:10.3969/j.issn.1007-9572.2013.05.090
人胃癌细胞原位移植转移裸鼠原发灶及转移灶中锌指蛋白139的表达及其与肿瘤侵袭转移的关系研究
Expression of ZNF139 and Its Relationship with Invasion and Metastasis in Primay Tumors and Metastases of Orthotopic Implantation Nude Mice
摘要
Abstract
Objective To investigate the expression of zinc finger protein 139 ( ZNF139 ) and its relationship with invasion and metastasis in the primary tumor and metastases of orthotopic implantation nude mice.Methods Orthotopic implantation nude mice with human gastric carcinoma cells were established.Then reverse transcription - polymerase chain reaction ( RT - PCR ) and immunohistochemical method ( S - P ) were used to detect the expression of ZNF139, MMP - 2 and MMP - 7 in orthotopic transplantation tumor, liver metastases, spleen metastasis, peritoneal metastasis and lymph node metastasis.Results The tumor formation rate was 93.3% ( 28/30 ), and hepatic metastases were 75.0% ( 21/28 ), spleen metastases were 67.9% ( 19/28 ), peritoneal metastases were 85.7% ( 24/28 ) and lymph node metastases were 39.3% ( 11/28 ) .The mRNA expression and protein positive expression rate of ZNF139 , MMP - 2 and MMP - 7 in liver metastases, peritoneal metastases and lymph node metastases were significantly higher than in primary tumor ( P < 0.05 ) .The expression of ZNF139 mRNA was positively correlated with the expression of MMP - 2 mRNA and MMP - 7 mRNA both in primary tumor and metastases ( P < 0.05 ).Conclusion Models of orthotopic implantation nude mice can well simulate the invasion and transfer process.Invasion and metastasis are stronger in metastases than in primary tumor, and ZNF139 may be involved in this process by promoting the expression of MMP -2 and MMP -7.关键词
胃肿瘤/原位移植模型/裸鼠/锌指蛋白139/侵袭/肿瘤/转移Key words
Stomach neoplasms/ Orlhopotic implantation model, nude mice/ Zinc finger protein 139/ Invasiveness,neoplasms/ Metastasis分类
医药卫生引用本文复制引用
赵群,李勇,范立侨,檀碧波,侯五辉,李兆星,王冬,刘羽..人胃癌细胞原位移植转移裸鼠原发灶及转移灶中锌指蛋白139的表达及其与肿瘤侵袭转移的关系研究[J].中国全科医学,2013,16(15):1740-1743,4.基金项目
国家自然科学基金资助项目(81072033) （81072033）
河北省自然科学基金资助项目(C2010000619) （C2010000619）
河北省科技厅科技支撑项目(11276103D-26) （11276103D-26）
河北省普通高校强势特色学科资助项目(冀教高[2005]52) （冀教高[2005]52）
