中国蔬菜Issue(24)：27-35,9.
番茄黄化曲叶病毒的系统发育分析及多重PCR快速检测
Systematic Development Analysis of Tomato Yellow Leaf Curl Virus and Multiplex PCR Rapid Detection
摘要
Abstract
Sequence alignment analysis was conducted on domestic Tomato yellow leaf curl virus ( TYLCV ) by DNA-A genomic sequence. The result of nucleotide sequence blast showed that the complete nucleotide sequence of various China isolates shared high identity from each other. Phylogenetic relationship analysis of TYLCV full genomic and coat protein nucleotide and amino acid sequence reveals that the middle eastern and coastal tomato production regions of China were infected by TYLCV-IL isolates. The Western inland areas and Southwestern inland areas were infected by TYLCCNV isolates. Gene polymorphism analysis showed that TYLCV had a strong conservative evolution, and the distribution of nucleotide mutations ( Pi) on the chromosome with high polymorphism. Therefore, according to strict conservative region of TYLCV, 3 pairs of specific primers were designed, and a high sensitive, high efficient and with high specificity multiplex PCR detection method of TYLCV was etablished.关键词
番茄黄化曲叶病毒/序列比对/系统发育/基因多态性/多重PCRKey words
Tomato yellow leaf curl virus/ Sequence blast/ Systematic development/ Gene polymorphism/ Multiplex PCR分类
农业科技引用本文复制引用
薛东齐,李景富,许向阳,姜景彬..番茄黄化曲叶病毒的系统发育分析及多重PCR快速检测[J].中国蔬菜,2012,(24):27-35,9.基金项目
"十二五"国家科技支撑计划项目(2012BAD02B00) （2012BAD02B00）
