中国兽医科学2013，Vol.43Issue(3)：233-237,5.
Dpl蛋白和PrP蛋白与Flotillins蛋白相互作用的酵母双杂交技术分析
Determination of interaction between Flotillins protein and Dpl protein/PrP by yeast two-hybrid system
摘要
Abstract
Priori protein (PrP) and Flotillins genes were amplified by RT-PCR from bovine brain tissue, and Dopple(Dpl) gene was amplified by RT-PCR from bovine testis tissue. Then,bait vector pGBKT7-Flotillin-l/2,prey vectors pGADT7-Dopple and pGADT7-PrP were constructed, and then co-transformed into AH109 yeast cells. The recombinant plasmids were no self-activation and no toxic to the host yeast cells. The results showed that PrP and Flotillins had a interaction while Dpi and Flotillins had no interaction in yeast cells.关键词
疯牛病/Flotillins蛋白/Dpl蛋白/酵母双杂交Key words
bovine spongiform encephalopathy/Flotillins protein/Dpl protein/yeast two-hybrid分类
农业科技引用本文复制引用
马俊俊,刘永生,丁耀忠,周建华,高宗良,陈豪泰,马丽娜,张杰..Dpl蛋白和PrP蛋白与Flotillins蛋白相互作用的酵母双杂交技术分析[J].中国兽医科学,2013,43(3):233-237,5.基金项目
国家自然科学基金资助项目(30671563,31072143) （30671563,31072143）
