中国兽医科学2013，Vol.43Issue(3)：252-255,4.
上海市猪源与人源耐甲氧西林金黄色葡萄球菌的分离及分子分型
Isolation and molecular typing of methicillin-resistant Staphylococcus aureus from swine and human in Shanghai
摘要
Abstract
The objective of this study was to investigate the prevalence and characteristic of methicillin-resistant Staphylococcus aureus (MRSA) isolated from swine and human in Shanghai. A total of 170 swine nasal swab samples were collected from three large-scale swine farms and one pig slaughterhouse. A total of 120 sputum and throat swab samples of human origin were collected from the patients in one hospital. MRSA were isolated and identified by using 75 g/L sodium chloride broth and CHROM agar and duplex PCR(nuc and mecA). The MRSA isolates were characterized by staphylococcal chromosomal cassette(SCC) mec typing,multilocus sequence typing (MLST) and spa typing. Among 83 swine S. aureus isolates, 45 strains were resistant and among 52 human S. aureus isolates, 15 were resistant. All MRSA isolates of swine origin were determined to be ST9-SCC mec IVb,and characterized as spa type t899(n = 37) and t2922 (n = 2). Fourteen of the 15 human MRSA isolates were determined to be ST5-SCC mec II -t002,and one was characterized as ST88(MLST)-tl0775(a novel spa type). The results showed that swine MRSA were ST9-t899-SCCmec IVb,and human MRSA were ST5-t002-SCC mec II in Shanghai. There were no signs of MRSA transmission between human and pig.关键词
耐甲氧西林金黄色葡萄球菌/多位点序列分型/葡萄球菌蛋白A分型/金黄色葡萄球菌盒式染色体分型Key words
methicillin-resistant Staphylococcus aureus(MRSA)/multilocus sequence typing(MLST)/spa typing/staphylococcal chromosomal cassette mec typing(SCCmec)分类
农业科技引用本文复制引用
王雪敏,姚建楠,李蓓蓓,赵秋华,杨传定,邵东华,王少辉,马志永..上海市猪源与人源耐甲氧西林金黄色葡萄球菌的分离及分子分型[J].中国兽医科学,2013,43(3):252-255,4.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(2012JB09) （2012JB09）
河北省教育厅自然科学重点项目(ZH200811) （ZH200811）
闵行区自然科学研究课题(2012MHZ003) （2012MHZ003）
