中国兽医科学2013，Vol.43Issue(3)：256-260,5.
非洲马瘟病毒VP7蛋白的原核表达及其间接ELISA方法的建立
Development of an indirect ELISA based on the prokaryotic expression of VP7 gene of African sickness virus
摘要
Abstract
To develop a method for specific detection of antibody of African horse sickness virus (AHSV), AHSV VP7 gene was optimized from GenBank and synthesized,and then transformed into Es-cherichia coli Rosetta(DE3). The results of SDS-PAGE and Western-blot revealed that the recombinant fusion VP7 protein was expressed in a high level and had good reactinogenicity. The purified recombinant VP7 protein was used as detection antigen to establish a ELISA for detection of antibody against AHSV. The reaction conditions in the indirect ELISA such as antigen coating concentration, the dilution of sera and reaction time of sera were optimized. Compared with the standard competitive ELISA, the coincidence rate between the two methods was 100%. The established indirect ELISA was then used to detect antibody against AHSV, which confirmed that the method was sensitive and specific.关键词
非洲马瘟病毒/VP7蛋白/间接酶联免疫吸附试验Key words
African horse sickness virus/VP7 protein/indirect ELISA分类
农业科技引用本文复制引用
潘佳亮,高利,相文华,戚亭,郭巍..非洲马瘟病毒VP7蛋白的原核表达及其间接ELISA方法的建立[J].中国兽医科学,2013,43(3):256-260,5.基金项目
公益性行业(农业)科研专项(201003075) （农业）
