中国兽医科学2013，Vol.43Issue(3)：266-270,5.
小反刍兽疫病毒M蛋白主要抗原表位区的原核表达及鉴定
Prokaryotic expression and identification of M protein main epitopes of peste des petits ruminants virus
摘要
Abstract
To prepare peste des petits ruminants virus(PPRV)-specific monoclonal antibody and monitor the clinical antibody level of PPRV, putative B-cell linear epitope sites of M protein of PPRV Nigeria75/1 strain was analysed by online software. A pair of primers was designed to amplify a 303 bp targeting gene fragment which was then cloned into the prokaryotic expression vector pET-32a( + ). The re-combinant protein was expressed in Escherichia coli BL21(DE3) by IPTG induction and purified. SDS-PAGE showed that the recombinant was expressed in the solube form with high expression level and Western-blot analysis indicated that the purified M protein possessed good reactinogenicity. The expressed M protein could be used for the preparation of specific monoclonal antibodies and surveillance of antibody level of PPRV.关键词
小反刍兽疫/M蛋白/抗原表位/原核表达Key words
peste des petits ruminants/M protein/epitope/prokaryotic expression分类
农业科技引用本文复制引用
黄华欣,李刚,史利军,赵占中,王勇,金红岩,李文超,陶春爱,隋修锟..小反刍兽疫病毒M蛋白主要抗原表位区的原核表达及鉴定[J].中国兽医科学,2013,43(3):266-270,5.基金项目
国家公益性行业科研专项(200903037) （200903037）
国家自然科学基金资助项目(31172342) （31172342）
