中国兽医科学2013，Vol.43Issue(3)：271-276,6.
鸡传染性法氏囊病病毒VP2基因密码子的优化及其在大肠杆菌中的表达
Codon optimization of VP2 gene of infectious bursal disease virus and its expression in Escherichia coli
摘要
Abstract
To improve the protein expression of VP2 of infectious bursal disease virus(IBDV) in Escherichia coli,VP2 gene structure of IBDV isolated from chicken with immunoprophylaxis defeat in Anhui was analyzed. There were 49 rare codons in the VP2 gene by contrast with codon usage in Escherichia coli, including 16 AGG/AGA(Arg) and 6 GGG(Gly). Two AGG were joined together (called box R)and some GGG codons were relative concentrate(called box G). The codon usage of wild type IBDV VP2 was optimized according to the codon bias of E. coli,and the full length optimized VP2 gene was synthesized,named mVP2, which was later cloned into the prokaryotic expression vectors pET-32a and pBV220 to construct recombinant expression vectors pET32-VP2 and pBV220-VP2 respectively. SDS-PAGE showed highly efficient expression of codon-modified IBDV VP2 gene in E. coli. Western-blot analysis showed that the expressed VP2 protein could react with the chicken against IBD serum. This study provided a basis for the studies of the structure and function of VP2 gene and IBD immune prevention and rapid diagnosis.关键词
鸡传染性法氏囊病病毒/VP2/密码子优化/表达Key words
infectious bursal disease virus/VP2/codon optimization/expression分类
农业科技引用本文复制引用
潘群兴,朱向东,欧阳伟,王晓丽,夏兴霞,毕振威,诸玉梅,董晨红,王永山..鸡传染性法氏囊病病毒VP2基因密码子的优化及其在大肠杆菌中的表达[J].中国兽医科学,2013,43(3):271-276,6.基金项目
国家自然科学基金资助项目(31272537) （31272537）
江苏省自然科学基金项目(BK2010471,BK2012787) （BK2010471,BK2012787）
江苏省农业科技自主创新基金项目[cx(10)426] （10）
