中国兽医科学2013，Vol.43Issue(3)：320-324,5.
黏菌素对小鼠肾毒性及其机制的研究
Nephrotoxicity induced by colistin and its mechanism in mice
摘要
Abstract
To investigate the toxic effect on kidney induced by colistin and its possible mechanism in mice,160 female mice were randomly divided into the high-dose group(15 mg/kg),middle-dose group(7. 5 mg/kg) ,low-dose group(3. 75 mg/kg) and the control group(9 g/L normal saline). Colistin sulfate was administered intravenously for 7 days. The blood BUN and Cre,the level of MDA and the activity of SOD in kidney and the semiquantitative score(SQS) of renal lesion were carried out on days 1,3,7 and 15. Compared with the control group, the body weights of mice treated by colistin reduced. On days 3 and 7, the level of blood BUN and Cre significantly increased, and the activity of SOD significantly decreased(P< 0. 01) while the content of MDA significantly increased(P<0. 01) ,the acute tubular damage with necrosis of the tubular epithelial cells occurred and the SQS increased to (2. 4 ± 0. 89), (3. 6±1. 14) (both P<0. 01) in the high-dose group. On day 7,in the middle-dose group,significant differences occurred in the levels of BUN,Cre and MDA and the activity of SOD, which had no significant difference in the low-dose group. On day 15,all the indexes presented a recovery tendency but the change of SOD and MDA were significantly different in the high-dose group and middle-dose group. The results revealed that the time-dependent and dose-dependent changes were induced in colistin induced-nephrotoxicity and the mechanism of oxidative stress may play an important role.关键词
黏菌素/肾毒性/时间依赖性/剂量依赖性/氧化应激Key words
colistin/nephrotoxicity/time-dependent/dose-dependent/oxidative stress分类
农业科技引用本文复制引用
代重山,李继昌,李广兴,李健..黏菌素对小鼠肾毒性及其机制的研究[J].中国兽医科学,2013,43(3):320-324,5.基金项目
国家自然科学基金资助项目(31272613) （31272613）
教育部留学回国人员科研启动基金项目(41) （41）
哈尔滨市科技创新人才研究专项资金留学回国人员项目(2012RFLXN005) （2012RFLXN005）
黑龙江省教育厅科学技术项目(12521043) （12521043）
