中国甜菜糖业Issue(1)：5-8,4.DOI:10.3639/j.issn.1002-0551.2013.01.002
红松松塔多糖三氯乙酸法脱蛋白工艺的研究
Deproteinization technology of polysaccharides from Pinus koraiensis with trichloroacetic acid method
摘要
Abstract
The deproteinization - technology of polysaccharides in pine Cone from Pinus koraiensis with TCA has been studied in this paper. Based on polysaccharide retention rate and deproteinization rate as evaluating indicators, the effects of sample solution/TCA ratio, shaking time and TCA concentration were studied. The experiment conditions were optimingized by orthogonal design, and the optimum conditions were obtained as follows: sample solution/TCA ratio was 1:3 ( v/v) , shaking time was 20 min and TCA concentration was 6%. The deproteinization rate was 72.41% . This study provided the theoretical basis for the production of polysaccharide of Pinus koraiensis.关键词
红松/松塔/多糖/脱蛋白/三氯乙酸法Key words
Pinus koraiensis/pine cone/polysaccharide/deproteinization technology/TCA method分类
轻工纺织引用本文复制引用
徐韧博,邹攀,杨鑫,蒙琦,黄微薇,胡兴龙,董爱军,王静..红松松塔多糖三氯乙酸法脱蛋白工艺的研究[J].中国甜菜糖业,2013,(1):5-8,4.基金项目
国家自然科学基金项目支持(资助号:31000831) （资助号:31000831）
黑龙江省青年科学基金项目支持(QC08C01) （QC08C01）
第四十五批中国博士后科学基金项目支持(20090450478) （20090450478）
哈尔滨工业大学科研创新基金项目支持(HIT.NSRIF.2008.30) （HIT.NSRIF.2008.30）
