中国药理学通报2013，Vol.29Issue(6)：778-782,5.DOI:10.3969/j.issn.1001-1978.2013.06.009
相关miRNAs对LS174T细胞中MRPs的影响
Effect of relative miRNAs on MRPs in LS174T cells
摘要
Abstract
Aim To investigate the transcriptional effect of hsa-miR-328 , hsa-miR-206 and hsa-miR-613 on multidrug resistance-associated protein ( MRP1 ,MRP3, and MRP5 ). Methods LS174T cells were transient transfected with pCMX-miR-328 , miR-206 , and miR - 613 expression vectors . Twenty - four hours after the treatment, mRNA level of MRP1 , MRP3 and MRP5 was detected by real-time PCR. Results The mRNA expression level of MRP3 was significantly inhibited by hsa-miR-328 in LS174T cells. The MRP1 mRNA expression was significantly inhibited by hsa-miR-206 in LS174Tcells. However, the mRNA expression level of MRP1 , MRP3 , and MRP5 was not influenced by has-mir-613. Conclusion Specific MRPs can be down-regulated after transfected with pCMX-miR-328 or 206 in LS174T cells.关键词
miRNA/MRP/基因调控/多药耐药/mRNA/转染Key words
miRNA/ MRP/ post-transcriptional regulation/ multidrug resistance/ mRNA/ transfection分类
医药卫生引用本文复制引用
应梦佳,毕惠嫦,胡冰芳,周许年,金晶,钟国平,黄民..相关miRNAs对LS174T细胞中MRPs的影响[J].中国药理学通报,2013,29(6):778-782,5.基金项目
国家"十二五"科技重大专项(No 2012ZX09506001-004) （No 2012ZX09506001-004）
广东省重点实验室项目(No 2011A060901014) （No 2011A060901014）
