中国药理学通报2013，Vol.29Issue(6)：814-817,4.DOI:10.3969/j.issn.1001-1978.2013.06.016
稳定表达α-Synuclein4种选择性剪接异构体的PC12细胞对神经毒性剂MPP+的敏感性研究
Study on the sensitivity of the PC12 cells expressing four α-synuclein isoforms to MPP+
摘要
Abstract
Aim To study the sensitivity of the PC12 cells expressing four α-syn isoforms to MPP+. Methods Four α-syn isoforms was cloned by PCR method, followed by subcloning into the pcDNA3. 1 eukaryotic expression vector. Four obtained recombination plas-mids were transfected into PC12 cells using Lipo-fectamine 2000 respectively. The protein expressions of these four isoforms were identified by WB. Results When exposed to MPP+ , these cell lines which over expressed exon 5-lacking form of a-syn isoforms showed the toxicity to PC12 cells. Conclusion The inhibition of alternative splicing to α-syn exon 5 can reduce the production of α-syn 112 and α-syn 98, which may be a good strategy for rescuing dopaminergic neurons from exposure to neurotoxic agents.关键词
α-synuclein/异构体/MPP+/PC12细胞/敏感性/帕金森病Key words
α-synuclein/ isoforms/ MPP+/ PC12 cells/ sensitivity/Parkinson's disease分类
医药卫生引用本文复制引用
马开利,宋连昆,苑玉和,张莹,陈乃宏..稳定表达α-Synuclein4种选择性剪接异构体的PC12细胞对神经毒性剂MPP+的敏感性研究[J].中国药理学通报,2013,29(6):814-817,4.基金项目
国家自然科学基金资助项目(No 81274122,81102831,81073078,30973887,U832008) （No 81274122,81102831,81073078,30973887,U832008）
国家科技重大专项(No 2012ZX09301002-004) （No 2012ZX09301002-004）
教育部创新团队,天然药物中活性物质的结构与功能研究(No IRT1007) （No IRT1007）
高等学校博士学科点专项科研基金新教师类资助课题(No 20121106120056) （No 20121106120056）
北京市重点实验,新的药物作用和药理评价(No BZ0150) （No BZ0150）
