中国药理学通报2013，Vol.29Issue(6)：867-870,4.DOI:10.3969/j.issn.1001-1978.2013.06.028
虎耳草抗前列腺癌生物活性部位筛选研究
Screening of anti-prostate-tumor parts from Saxifraga stolonifera
摘要
Abstract
Aim To screen the anti-prostate-tumor parts from Saxifraga stolonifera. Methods MTT assay was used to detect anti-prostate-tumor activity of each extraction. The cell ratio in the different stages of the cell cycle and apoptotic ratio of PC-3 cells were detected by flow cytometry( FCM ). Electron microscopy was used to observe the morphological features of PC-3 ap-optosis. Results Different extract fractions had certain inhibition on the proliferation of PC-3 cells. The effect was enhanced with the increase in drug concentration and time. Conclusion Ethanol extracted with ethyl acetate extract has good anti-prostate -tumor activity.关键词
虎耳草/前列腺癌/活性部位/MTT法/PC-3细胞/筛选Key words
Saxifraga stolonifera/ prostate-tumor/ activity parts/MTT Method/PC-3 cell/screen分类
医药卫生引用本文复制引用
周欣,陈华国,黄志金,杨世林,杨占南..虎耳草抗前列腺癌生物活性部位筛选研究[J].中国药理学通报,2013,29(6):867-870,4.基金项目
贵州省中药现代化科技产业研究开发专项项目[黔科合中药字(2010)5023号] （2010）
贵州省科技创新人才团队建设项目[黔科合人才团队(2011)4008] （2011）
贵阳市科学技术计划项目[(2010)筑科合同字第1-中-23号] （2010）
贵州省教育厅特色重点实验室项目[黔科合KY(2012)005号] （2012）
