植物研究2013，Vol.33Issue(3)：339-345,378,8.DOI:10.7525/j.issn.1673-5102.2013.03.014
纳米紫杉醇在人源肠Caco-2细胞模型的吸收转运研究
Nano-paclitaxel Transport by Human Intestinal Epithelial Caco-2 Cells
摘要
Abstract
Many studies had proved that oral administration of paclitaxel was poorly absorbed and easy to cause hypersensitivity reactions.For this reason,we produced a new nano-paclitaxel by paclitaxel granule wrapped with mannitol and Human Serum Albumin ( HSA),which had better water solubility and lower systemic toxicity.In order to research the absorption of nano-paclitaxel after oral administration,the transepithelial flux of nanopaclitaxel was studied using the human colonic cell line Caco-2 cells.The flux of nano-paclitaxel ( at a concentration range of 0.5 - 20 μmol .L-1) across the Caco-2 cell layer was linear with time for up t0 3 h.The apparent permeability coefficient ( Papp) for the apical to basolateral transport of nano-paclitaxel ( 20.9±2.1 ×10-6 cm · s-1; N =3) and the apparent maximal concentration ( KM) of the active efflux component ( 14.00μLmol·L-1) were relatively high,suggesting that nano-paclitaxel may have a better absorption rate in the intestinal tract.关键词
纳米紫杉醇/Caco-2细胞模型/体外转运吸收/P-gp糖蛋白Key words
nano-paclitaxel/Caco-2 cell/transport absorption/P-glycoprotein分类
农业科技引用本文复制引用
徐超,姜守刚,龚显峰,祖元刚..纳米紫杉醇在人源肠Caco-2细胞模型的吸收转运研究[J].植物研究,2013,33(3):339-345,378,8.基金项目
The Special Fund for Forestry Scientific Research in the Public Interest (201204601),the Fundamental Research Funds for the Central Universities (DL12EA01-03) （201204601）
