医学分子生物学杂志2013，Vol.10Issue(2)：91-94,4.DOI:10.3870/j.issn.1672-8009.2013.02.006
大鼠皮层线粒体双向凝胶电泳体系的建立
Establishment of Two-Dimensional Gel Electrophoresis System for Rat Cortex Mitochondria
摘要
Abstract
Objective To establish two-dimensional gel electrophoresis system for rat cortex mitochondria. Methods The adult male SD rats were sacrificed by cervical dislocation , the brain tissues quickly harvested and thecortex isolated on ice. The mitochondria were extracted and purified twice by density gradient centrifugation. Two-dimensional gel electrophoresis was performed. Immobilized pH gradient isoelectric focusing (IPG-IEF) was modified and carried out. The gels were focused at 3500 V for a total of 14000Vh and then subjected to SDS-PAGE and silver staining. Results Electrophoregram of rat cerebral cortex mitochondria was obtained with high resolu -tion by using the established two -dimensional gel electrophoresis system. Conclusion The two-dimensional gel electrophoresis system for rat cortex mitochondria was successfully established , which provides a theoretical basis and a technical support for research of cerebral cortex mitochondrion pro -teins in diseases.关键词
蛋白质组学/双向凝胶电泳/线粒体Key words
proteomics/ two dimensional gel electrophoresis / mitochondria分类
医药卫生引用本文复制引用
杨涌涛,谢鹏,陈康宁,黄河清,贺伟峰..大鼠皮层线粒体双向凝胶电泳体系的建立[J].医学分子生物学杂志,2013,10(2):91-94,4.基金项目
国家重点基础研究发展规划项目(973计划)(No.2009CB918300) （973计划）
