中国临床医学2012，Vol.19Issue(5)：459-461,3.
雷帕霉素和紫杉醇对骨髓内皮祖细胞数量及功能的影响
Effects of Rapamycin and Paclitaxel on the Number and Activity of Bone Marrow-Derived Endothelial Progenitor Cells
摘要
Abstract
Objective:To investigate the effects of rapamycin and paclitaxel on the number and activity of bone marrow - derived endothelial progenitor cells(EPCs). Methods: Bone marrow mononuclear cells were isolated from rat bone marrow by centrifu-gation and they were cultured on gelatin - coated dishes. After 7- day culture, adherent cells were treated with rapamycin or paclitaxel in a series of concentrations (0. 1μg/mL, 1μg/mL, and 5 μg/mL) for 24 h. EPCs were identified as adherent cells double positive for DiI - acLDL - uptake and lectin binding by direct fluorescent staining. Proliferation and migration of EPCs were determined using MTT assay and modified version of the Boyden chamber assay, respectively. EPCs adhesion assay was performed by replanting the cells on gelatin- coated dishes, and then adherent cells were counted. Results: Incubation of EPCs with rapamycin or paclitaxel decreased the number of EPCs. Rapamycin and paclitaxel also decreased the proliferative, migratory, and adhensive capacity of EPCs in a concentration - dependent manner(P<0. 05). At the same time, the influence of the same concentrations of rapamycin and paclitaxel on the number and function of EPCs showed no significant differences. Conclusions: Rapamycin and paclitaxel decreased the number, as well as the proliferative, migratory, and adhensive capacity of EPCs.关键词
雷帕霉素/紫杉醇/内皮祖细胞/细胞培养Key words
Rapamycin/ Paclitaxel/ Endothelial progenitor cells/ Cell culture分类
医药卫生引用本文复制引用
朱琳琳,陈绍良,张娟,刘志忠..雷帕霉素和紫杉醇对骨髓内皮祖细胞数量及功能的影响[J].中国临床医学,2012,19(5):459-461,3.基金项目
江苏省自然科学基金(编号:BK2009044) （编号:BK2009044）
